| 1. | Sr | | Dylan Hartnett | 47.41a | KS | Wichita State | NCAA Division I Prelims - West Regionals | Thu, May 24 |
| 2. | So | | Kevin Piraino | 47.73a | IN | Indiana State | MVC Outdoor Championships | Sun, May 13 |
| 3. | Fr | | Alex Reed | 47.99a | KS | Wichita State | MVC Outdoor Championships | Sun, May 13 |
| 4. | Fr | | Sheldon Magee | 48.02a | IA | Northern Iowa | MVC Outdoor Championships | Sun, May 13 |
| 5. | Fr | | Jesse Davenport | 48.08a | IA | Northern Iowa | MVC Outdoor Championships | Sun, May 13 |
| 6. | So | | Coty Kramer | 48.09a | IL | Illinois State | MVC Outdoor Championships | Sun, May 13 |
| 7. | Sr | | Aaron Young | 48.46a | KS | Wichita State | Drake Relays 2012 | Sat, Apr 28 |
| 8. | So | | DJ Lindsey | 48.80a | KS | Wichita State | MVC Outdoor Championships | Sun, May 13 |
| 9. | So | | Mike Flores | 49.04a | KS | Wichita State | K.T. Woodman Classic | Sat, Apr 14 |
| 10. | So | | Austin Bahner | 49.05a | KS | Wichita State | Drake Relays 2012 | Sat, Apr 28 |
| 11. | Sr | | Shaun James | 49.62a | IA | Drake | MVC Outdoor Championships | Sun, May 13 |
| 12. | Sr | | Justin Hickey | 49.66a | KS | Wichita State | MVC Outdoor Championships | Sun, May 13 |
| 13. | So | | Brett Wright | 49.83a | IA | Drake | Stanford Invitational | Sat, Apr 7 |
| 14. | Fr | | Ryan Dickson | 49.94c | IN | Indiana State | Billy Hayes Invitational | Fri, May 4 |
| 15. | Jr | | Daniel Gooris | 50.16a | IA | Northern Iowa | MVC Outdoor Championships | Sun, May 13 |
| 16. | Fr | | Ryan Henrickson | 50.24c | IN | Indiana State | Billy Hayes Invitational | Fri, May 4 |
| 17. | So | | Tobias Boese | 50.51a | KS | Wichita State | MVC Outdoor Championships | Sun, May 13 |
| 18. | So | | Ryan Hocker | 50.80a | KS | Wichita State | Don Kirby Invitational | Sat, Apr 7 |
| 19. | Jr | | Mac Griffith | 50.98a | KS | Wichita State | MVC Outdoor Championships | Sun, May 13 |
| 20. | Jr | | Alex Freshour | 51.27a | KS | Wichita State | MVC Outdoor Championships | Sun, May 13 |
| 21. | - | | Dylan Filburn | 51.31a | KS | Wichita State | Don Kirby Invitational | Sat, Apr 7 |
| 22. | Fr | | Remy Lewis | 51.34c | IN | Indiana State | Billy Hayes Invitational | Fri, May 4 |
| 23. | - | | Ebo Browne | 52.30a | KS | Wichita State | Don Kirby Invitational | Sat, Apr 7 |
| 24. | Jr | | Robert Webb | 52.94a | IN | Indiana State | MVC Outdoor Championships | Sun, May 13 |
| 25. | Fr | | Dean Jordan | 53.12a | IL | Southern Illinois-Carbondale | MVC Outdoor Championships | Sun, May 13 |
| 26. | So | | Ben Lindaman | 53.37a | IA | Northern Iowa | MVC Outdoor Championships | Sun, May 13 |
| 27. | Fr | | Kevin Fisch | 54.90a | IL | Illinois State | MVC Outdoor Championships | Sun, May 13 |
| 28. | Jr | | Tyler Clark | 55.04a | IA | Northern Iowa | Drake Relays 2012 | Sat, Apr 28 |