| 1. | - | | Relay Team | 4:04.50 | CA | Walter Woodward Elementary | MUST 4-6th Trials | Tue, May 1 |
| 2. | - | | Bridger Blakeslee
Reggie Duke
Teyano Tweedie
Dylan Justesen | 4:15.14a | ID | Sandcreek | Upper Valley Conference | Tue, May 8 |
| 3. | - | | Hayden Livingston
Darin Nielsen
Chance Briggs
Jace Ritchie | 4:16.13a | ID | Rigby | Upper Valley Conference | Tue, May 8 |
| 4. | - | | Trejo Luis
Marlin Nathaniel
Rapp Braxton
Resendez Brenden | 4:17.03a | ID | Rocky Mountain | Upper Valley Conference | Tue, May 8 |
| 5. | - | | Marcus Gillespie
David Searle
Austin Stewart
Keigan Henderson | 4:19.01a | ID | Hobbs | Upper Valley Conference | Tue, May 8 |
| 6. | - | | Conner Andrews
Nathan Gerdes
Trayjen Miller
Clay Elkington | 4:23.00 | ID | Sugar-Salem | Frontier 3A, Sgr, SoF, Ttn, Sal | Thu, Apr 19 |
| 7. | - | | Grant Robinson
Herbert Padilla
Preston Laws
Gianmarco Terrones | 4:24.05a | VA | Potomac | SSSAS MS Home Meet #1 | Thu, Apr 19 |
| 8. | - | | Jared Hensley
Carson Cline
Jacob Bolinger
Dillon Bennett | 4:27.80 | IA | Lenox | East Union MS Little Eagle Relays | Fri, May 11 |
| 9. | - | | B Spaulding
Evan McCall
B Brighton
C Mecham | 4:28.06a | ID | Eagle Rock | Upper Valley Conference | Tue, May 8 |
| 10. | - | | Jackson Mikkelsen
Joey Huntington
Jesse Johnson
Alex Synacek | 4:28.40 | IA | Orient-Macksburg | Bedford MS Invite | Mon, Apr 30 |
| 11. | - | | Mason Marsh
Josh Parra
Alex Salazar
Jacey Shaul | 4:28.60 | NE | Gering | Gering Middle School Relays | Sat, Apr 21 |
| 12. | - | | Micah Mudlaff
Nate Herbers
Nathan Hawley
Ian Whitmore | 4:29.20 | IA | Des Moines Christian | Panorama JH Relays | Tue, Apr 10 |
| 13. | - | | Grant Gordon
Carder Phillips
Austin Cox
Ty Thomas | 4:30.40a | IA | Van Meter | Van Meter JH CO-ED | Thu, Apr 12 |
| 14. | - | | Relay Team | 4:31.50a | CA | Stella Brockman | MUST 7-8th Trials | Wed, May 2 |
| 15. | - | | Relay Team | 4:34.70 | IA | Mt Ayr | POI West MS Meet | Thu, Apr 26 |
| 16. | - | | Ryan Jensen
Noah Cooper
Heath Downing
Tim Settlemoir | 4:34.80 | IA | Nodaway Valley | Nodaway Valley MS Invitational | Mon, Apr 23 |
| 17. | - | | Blayne Godshall
Jordan Uselton
Andrew Hartzler
Micah Ruiz Esparza | 4:41.00 | MO | Summit Christian Academy | Jr. Viking Relays | Mon, Apr 16 |
| 18. | - | | Relay Team | 4:42.40 | DC | Georgetown Day | Holton Arms Meet | Thu, May 10 |
| 19. | - | | Nick Johnston
Brandyne Olin
Jake Leeps
Ian Showers | 4:42.78a | IA | Clarke Community | MS Rebel Raider Relays | Tue, Apr 17 |
| 20. | - | | Relay Team | 4:44.00 | CA | Neil Hafley Elementary | MUST 4-8th Finals | Tue, May 8 |
| 21. | - | | Massimo (Max) Eichner
Eric Matzelevich
Alexander Brown
Aryemis Brown | 4:44.20 | MD | Bullis | IAC Middle School Championships | Mon, May 21 |
| 22. | - | | Taylor O'Dea
Brennon McCuddin
Jaylan White
Wesley Bedwell | 4:44.40 | IA | Interstate 35 | Nodaway Valley MS Invitational | Mon, Apr 23 |
| 23. | - | | Hayden Ogg
Cade Pelzer
Wyatt Robinette
Jacob Reynolds | 4:44.60 | IA | Griswold | Nodaway Valley MS Invitational | Mon, Apr 23 |
| 24. | - | | Relay Team | 4:45.50 | CA | Nile Garden | MUST 4-8th Finals | Tue, May 8 |
| 25. | - | | Tim Draper
Ben Bennett
Cody Hansen
Gavin Lamb | 4:46.20 | IA | West Central Valley | Nodaway Valley MS Invitational | Mon, Apr 23 |
| 26. | - | | Relay Team | 4:46.50 | MD | Landon | Holton Arms Meet | Thu, May 10 |
| 27. | - | | Relay Team | 4:46.98a | DC | St Albans | SSSAS MS Home Meet #1 | Thu, Apr 19 |
| 28. | - | | Tyson Schwanz
Steven Wittrock
Stephan Wehtje
Marcus Fritton | 4:47.10 | NE | Gothenburg | Gothenburg Dutch Zorn relays | Thu, Apr 26 |
| 29. | - | | Relay Team | 4:48.72a | ID | Salmon | Salmon MS Meet | Fri, Apr 27 |
| 30. | - | | Relay Team | 4:49.00 | ID | Firth | Firth MS meet | Tue, Apr 17 |
| 31. | - | | Raven Williamson
Wes Erickson
Lucas Larsen
Tyler McAfee | 4:49.20 | IA | CAM | Nodaway Valley MS Invitational | Mon, Apr 23 |
| 32. | - | | Brock Beeler
Sam Courtney
Jacob Way
T.J. Wickland | 4:49.60 | IA | Guthrie Center | Nodaway Valley MS Meet | Mon, Apr 16 |
| 33. | - | | Garrison Antoine
Quentin Siefkas
Caleb Miller
Dustin Miller | 4:50.80 | IA | Murray | East Union MS Little Eagle Relays | Fri, May 11 |
| 34. | - | | Relay Team | 4:51.20 | CA | Joshua Cowell | MUST 4-8th Finals | Tue, May 8 |
| 35. | - | | Tyler Davis
Cameron Thrope
Jaden Gillespie
Will Greenwood | 4:55.00 | IA | Lamoni | East Union MS Little Eagle Relays | Fri, May 11 |
| 36. | - | | Relay Team | 4:56.00 | ID | North Fremont | Firth MS meet | Tue, Apr 17 |
| 37. | - | | Paker Higbee
Boline Avery
Hunter Deuel
Ethan Clarke | 4:57.00a | ID | Taylorview | Upper Valley Conference | Tue, May 8 |
| 38. | - | | Chance Masters
Ben Savely
Kade Kelso
Rayden Boswell | 4:57.40 | IA | Central Decatur | East Union MS Coed Invitational | Tue, Apr 24 |
| 39. | - | | Zane Corrigan
Jason Rembelski
Ty Redick
Jack Peninger | 4:57.46a | ID | Challis | Salmon MS Meet | Fri, Apr 27 |
| 40. | - | | Relay Team | 5:02.30 | DC | Alice Deal | Landon Home Meet | Tue, Apr 24 |
| 41. | - | | Relay Team | 5:04.70a | CA | McParland | MUST 7-8th Trials | Wed, May 2 |
| 42. | - | | Brian Vanderheiden
Josh Groves
Clay Reynolds
Sterling Bean | 5:06.70 | IA | Earlham | Nodaway Valley MS Meet | Mon, Apr 16 |
| 43. | - | | Isaiah Brooks
Christian Miller
Brockton Troutman
Casey Bree | 5:07.00 | CA | Central | Mt. Sac Youth Days | Fri, Mar 30 |
| 44. | - | | Kobe Adolph
Dylan Varghese
Chuck O'Brien
Eric Karsten | 5:07.40 | VA | St Stephens and St Agnes | IAC Middle School Championships | Mon, May 21 |
| 45. | - | | Relay Team | 5:08.40 | DC | Washington Latin | Holton Arms Meet | Thu, May 10 |
| 46. | - | | Relay Team | 5:08.70 | CA | Veritas Elementary | MUST 4-8th Finals | Tue, May 8 |
| 47. | - | | Quentin Sefrit
Robert Hitch
Cle Beason
Wyatt Dillon | 5:10.58a | IA | Bedford | MS Rebel Raider Relays | Tue, Apr 17 |
| 48. | - | | Relay Team | 5:26.00 | ID | Ririe | Firth MS meet | Tue, Apr 17 |
| 49. | - | | Andrew Carlson
Jon Spunaugle
Jared Mattson
Ricky Hensley | 5:26.40 | IA | Villisca | Bedford MS Invite | Mon, Apr 30 |
| 50. | - | | Johnathan Kinser
Jerad Shires
Jacob Kinyon
Dalton Davis | 5:57.90 | IA | Corning | East Union MS Coed Invitational | Tue, Apr 24 |
