| 1. | - | Tierra Hubbard
Tiarra Booker
Jessica Robertson
Tiffany Villarreal | 50.48a | Appomattox County | |
| 2. | - | Destinee Mitchell
Amber Clements
Jessica Myers
Bricey Rushing | 50.98a | Gretna | |
| 3. | - | Margarita Shevchenko
Tara Holman
Lauren Bowling
Alexandra Hairston | 51.20a | George Mason Middle Sr | |
| 4. | - | Tylisha Crockett
Justice Gordon
Nia Jones
Rashauna Carter | 51.22a | Northumberland | |
| 5. | - | Shamra Wilkerson
Elaine Atkins
Morgan Rudd
Ashley Eggleston | 51.65a | King William | |
| 6. | - | DeAnna Hedgepeth
Tyra Hedgepeth
Jordan Quinn
Makala Johnson | 51.74a | George Wythe | |
| 7. | - | Aariana Lane
Mikayla Lockett
Robin Younger
Kenisha Staten | 52.00a | William Campbell | |
| 8. | - | Anijza Hill
NaKimbra Curry
Rachel Chan
Tianna Woodson | 52.08a | Manassas Park | |
| 9. | - | Ay'Sha Wesley
Britnee Terry
Alexis Oliver
Bria Long | 52.09a | Bluestone Sr | |
| 10. | - | Sarah Thibodeau
Madison Whitesell
Chiara Gabellieri
Melanie Meadows | 52.35a | Wilson Memorial | |
| 11. | - | Alayah Eccleston
Chelsea Butler
Brianna Simpson
Tionna Austin | 52.49a | Virginia | |
| 12. | - | Timmesha Stringfield
Ricki Parham
Briana Ellis
Ebonie Byrd | 52.57a | Sussex Central | |
| 13. | - | Laura Puckett
Tess Carper
Riley Harvey
Jada Penn | 53.92a | Radford | |
| 14. | - | Megan Yopp
Rene Jones
Neileen Shoemaker
Savanna Burton | 54.18a | Narrows | |
| 15. | - | Bailie Light
Jordan Gose
Destiny Hill
Morgan Gose | 54.48a | Gate City | |
| 16. | - | Lindsay Lawson
Samantha Cole
Erin Stanley
Kala Morgan | 54.96a | Eastside | |
| 17. | - | Tiffany Spencer
Kassie Conway
Deysia Ayres
Johniesha Smith | 55.00a | Buckingham County | |
| 18. | - | Sherrece Baker
Viola Thompson
De'Shonda Gaskins
Brittany Greene | 55.81a | Essex | |
| 19. | - | Tayanna Lee
Amelia Walker
Iman Lee
Tori McKenzie | 56.84a | Chatham | |
| -- | - | Shalonda Green
Makhalia Bibbs
Tess Lee
Destiny Johnson | DQ | West Point | |
| 1. | - | Marjorie Grumbine
Anne Grumbine
Alyssa Ford
Reanna Hamm | 4:07.53a | James River (Buchanan) | |
| 2. | - | Jen Owens
Shaina Williams
Taylor Limer
Victoria Dumas | 4:09.10a | Manassas Park | |
| 3. | - | DeAnna Hedgepeth
Tyra Hedgepeth
Meredith Willis
Jordan Quinn | 4:10.75a | George Wythe | |
| 4. | - | Deonna Jennings
Amber Clements
Bricey Rushing
Jessica Myers | 4:16.59a | Gretna | |
| 5. | - | Shalonda Green
Tess Lee
Destiny Johnson
Jenna Roane | 4:16.98a | West Point | |
| 6. | - | Kirsten Campbell
Hannah Clinedinst
Marissa Sager
Katlyn Ayers | 4:17.48a | Strasburg | |
| 7. | - | Rachel Townsend
Madison Whitesell
Sarah Conyers
Chiara Gabellieri | 4:19.01a | Wilson Memorial | |
| 8. | - | Autumm Jackson
Christin Thomas
Maranda Moss
Kayla Issac | 4:19.16a | King William | |
| 9. | - | Ashton Davis
Christina Dinges
Amanda Dotson
Emily Jordan | 4:20.10a | Stonewall Jackson (Q... | |
| 10. | - | Megan Waring
Amanda Spain
Natalie Flagg
Channing Alsberry | 4:20.22a | Clarke County | |
| 11. | - | Emily Muncy
Chandra Goodson
Sarah Phillips
Savannah Hulbert | 4:20.48a | Floyd County | |
| 12. | - | Emily Silkie
Shelby Hudson
Beard Meghan
Katy Patterson | 4:23.88a | Parry McCluer | |
| 13. | - | Ali Morris
Mackenzie Arney
Morgan Miles
Lynn Wright | 4:32.65a | Lee | |
| 14. | - | Christine Robbins
Jessica Nelson
Sabrina Myers
Mariah Davis | 4:39.29a | Mathews | |
| 15. | - | Bailie Light
Jordan Gose
Ashley Carter
Destiny Hill | 4:43.77a | Gate City | |
| 16. | - | Sidney Jackson
Madison Brooks
Jazmine Vanover
Caroline Taylor | 4:48.38a | Central (Wise) | |
